ATRA與Am80誘導HL-60分化的調控機制：微型核醣核酸與基因網路
The regulatory mechanism of ATRA- and Am80-induced HL-60 cell differentiation: miRNA and gene network
Date Issued
2007
Date
2007
Author(s)
Chen, Cheng-Pao
DOI
zh-TW
Abstract
All-trans retinoic acid (ATRA) and Am80, natural and synthetic derivatives of Vitamin A, have been used in oncology for many years and are useful for inducing HL-60 to differentiate to granulocyte. In this study, we showed that TGF-β signaling pathway is the most perturbed and involved in differentiation process using microarray and bioinformatics approach. With time-series gene expression profiles measured by the quantitative real-time PCR, we constructed a gene network of ATRA- and Am80-induced human leukemia cell line HL-60 differentiation. The results from comparative analysis of target genes indicated that this gene network was regulated by some candidate microRNAs. MicroRNAs are non-coding, single-stranded RNAs of 20 ~ 25 nucloetides that execute a gene regulator function responsible for cell growth, cell differentiation, disease, and cancer. In our results, the expression of miR-96 and miR-107 were significantly induced by ATRA and Am80. In summary, our results showed that miR-96 and miR-107 seem to be the regulators involved in the gene network of leukemia cell differentiation.
Subjects
ATRA
Am80
HL-60
血癌
基因網路
微型核醣核酸
Leukemia
Gene network
miRNA
SDGs
Type
other
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