Construction of an inducible transposable element to terminate selectable marker gene
Date Issued
2007
Date
2007
Author(s)
Tai, Hung-Kuang
DOI
zh-TW
Abstract
Selectable marker genes (SMGs) are often necessary during the process of plant transformation, but unnecessary once transgenic plants have been obtained. Besides, SMGs is involved in biosafety. Therefore, in this work, an inducible transposable element was constructed to terminate the selectable marker gene：modified EPSPS gene. Firstly, the 3’ end of the Ac was inserted in the first intron of the modified rice EPSPS gene, which triggered by the nos promoter. Secondly, The 3’ end of the Ac was inserted in the fourth intron of the inducible Ac transposase gene trigged by PR-1a promoter. Thus, this inducible transposable element contains not only the exon E of Ac transposase gene but also the first exon of the modified EPSPS gene and its promoter. This construct, which termed as Cpt5ne3, was introduced into rice, tobacco, and arabidopsis. The modified EPSPS gene can be transcribed normally in all three species and allow the transgenic rice and arabidopsis to be Glyphosate-resistant. Also, the transposase gene is transcribed correctly. Therefore, it is expected that transposition event can be induced by salicylic acid
Subjects
嘉磷塞
篩選標記基因
轉位子
EPSPS
轉位
glyphosate
selectable marker genes
transposable element
Type
thesis
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