Identification of Nuclear Localization Signal (NLS) in PTPN22
Date Issued
2010
Date
2010
Author(s)
Sun, Yi-Wei
Abstract
PTPN22 is a protein tyrosine phosphatase that participates in the negative regulation of T cell signaling in cytoplasm. Recently, our lab shows that PTPN22 could locate inside the nucleus. However, the molecular mechanism of PTPN22 in nuclear import is poorly understood. Proteins that import from the cytosol to the nucleus contain basic amino acids-rich nucleus localization signal (NLS). We demonstrated Region C of PTPN22 was a potential NLS and necessary for nuclear import. Cells transfected with fluorescent vectors encoding Region C-deleted PTPN22 showed clear nuclear distribution. Mutation analysis showed A and B mutations of PTPN22 are critical sites for nuclear import of PTPN22, because the Mutant A and B impaired the capability of nuclear transport of PTPN22. To further identify sequences responsible for nuclear import of PTPN22, Region I or/and Region II was fused with double GFP and examined their abilities to target GFP into the nucleus. The results showed that Region I of PTPN22 could import GFP into the nucleus. Our data indicated that the Region I of PTPN22 served as NLS and played a vital role in nuclear import.
Subjects
PTPN22
NLS
Type
thesis
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