Glycated matrix up-regulates inflammatory signaling similarly to Porphyromonas gingivalis lipopolysaccharide
Journal
Journal of Periodontal Research
Journal Volume
48
Journal Issue
2
Pages
184-193
Date Issued
2013
Author(s)
Abstract
Background and Objective: Hyperglycemia and advanced glycation end-products (AGEs) have been hypothesized as the etiologic factors of diabetic periodontitis. The aim of this study was to clarify in greater detail the patterns of AGE-mediated periodontal inflammation under various physiological conditions. Material and Methods: The deposition of AGEs and expression of the receptor for AGEs (RAGE) were identified by immunohistochemistry in Sprague-Dawley rats with experimentally induced periodontitis or diabetes. Human periodontal ligament cells (PDLCs) and mesenchymal stem cells (MSCs) were cultured under simulated conditions of hyperglycemia, Porphyromonas gingivalis lipopolysaccharide (LPS) stimulation and matrix glycation. Cell viability and expression of toll-like receptors (TLRs), Rage, an inflammatory signaling initiator (nuclear factor kappa light chain enhancer of activator β cells), an oxidative stressor (heme oxygenase-1) and collagen synthesis (type I and type IV) genes were evaluated. Results: The deposition of AGEs and the expression of Rage were evident in the inflamed periodontal tissues in all rats and appeared to be enhanced in rats with diabetes. Matrix glycation augmented cytotoxicity, up-regulated RAGE and TLRs in both PDLCs and MSCs, and significantly activated downstream inflammatory signaling in MSCs. Oxidative stress was significantly increased under matrix glycation in both PDLCs and MSCs and was significantly increased at a high-glucose concentration in MSCs. A consistent decrease in expression of type I and type IV collagens was observed in MSCs, but a delayed reduction was noted in PDLCs. Conclusions: Matrix glycation modulated cell behavior to induce inflammation equivalent to that produced by incubation with P. gingivalis LPS. Periodontal inflammation also led to matrix glycation, thus demonstrating a definite interaction between diabetes and periodontitis. ? 2012 John Wiley & Sons A/S.
SDGs
Other Subjects
advanced glycation end product; advanced glycosylation end product receptor; advanced glycosylation end-product receptor; collagen type 1; collagen type 2; heme oxygenase 1; immunoglobulin enhancer binding protein; immunoglobulin receptor; lipopolysaccharide; NFKB1 protein, human; streptozocin; toll like receptor; alveolar bone loss; animal; article; cell culture; cell survival; comparative study; cytology; experimental diabetes mellitus; human; hyperglycemia; immunology; male; mesenchymal stroma cell; oxidative stress; pathology; periodontal ligament; periodontitis; physiology; Porphyromonas gingivalis; rat; signal transduction; Sprague Dawley rat; upregulation; Alveolar Bone Loss; Animals; Cell Survival; Cells, Cultured; Collagen Type I; Collagen Type II; Diabetes Mellitus, Experimental; Glycosylation End Products, Advanced; Heme Oxygenase-1; Humans; Hyperglycemia; Lipopolysaccharides; Male; Mesenchymal Stromal Cells; NF-kappa B p50 Subunit; Oxidative Stress; Periodontal Ligament; Periodontitis; Porphyromonas gingivalis; Rats; Rats, Sprague-Dawley; Receptors, Immunologic; Signal Transduction; Streptozocin; Toll-Like Receptors; Up-Regulation
Type
journal article