The Effect on Yeast Growth, on Acute Toxicity and Teratogenesis of High Dose Purified Montmorillonite in Rats and Pregnant Rats
Date Issued
2004
Date
2004
Author(s)
Feng, Yi-Kai
DOI
zh-TW
Abstract
To continue the examine of using montmorillonite to be a anticancer drug carrier of 5-FU, and its application of the replacement in pharmacology; In this study, we used a safe and non-pathological microorganism, Saccharomyces cerevisiae, to be our experimental model administrated with different dose of montmorillonite, montmorillonite-K6Fe(CN)3, or montmorillonite-K6Fe(CN)3-sodium alginate to proceed some toxicological assays. As a result of different formulas of montmorillonite, it could either enhance or inhibit the growth of Saccharomyces cerevisiae; to prove this, we found that the best formula, montmorillonite-K6Fe (CN)3-sodium alginate which was based on the dose-response analysis, could effectively inhibit the growth of Saccharomyces cerevisiae at a appropriate dosage.
On the other hand, the chemical structure of montmorillonite has been studied extensively but it dose not understand completely, especially the toxicity of montmorillonite to creature is still unclear. In this study, we used rats to proceed in vivo animal experiments to examined the acute toxicological effects of montmorillonite within 72 hours. Experimental animals were administrated with 142.9 mg/Kg of montmorillonite solution, and intravenous injected the rats with 14.29 mg/Kg of montmorillonite solution, respectably. And, the vehicles were only treated with PBS buffer. Each group of experimental animals were eight rats. After drug treatment, we observed their clinical symptoms and mortal conditions and we also collected the blood samples of these rats to carrier out blood routine examination included CBC and biochemistry analysis. Moreover, we sacrificed these rats and then took their organs out for examinating histochemistry sections included heart, liver, spleen, lung and kidney. The data showed the rats treated with oral montmorillonite were significantly increasing in Hb, Haematocrete and RBC counting than those with oral PBS buffer (P<0.05). Hoverer, MCV, MCH, MCHC, WBC Count and WBC differentiation analysis were no difference between experimental animals and vehicles (P＞0.05). The same result was reveled in each hematological analysis in the intravenous injected rats and vehicles (P＞0.05). From the results of biochemistry analysis, there was a decrease in the level of plasma sodium and chloride (P<0.05) and a notable decrease in the plasma calcium (P<0.01) in oral montmorillonite rats than that in vehicles. On the contrary, those intravenous injected montmorillonite were compared to vehicles, we found that ALT (SGPT) was significantly increasing (P<0.05), and plasma potassium was extremely deceasing (P<0.01) than the normal control. It deserves to be mentioned that histochemical examinations were all normal indicating montmorillonite may without any toxicity, but it may not suitable for the patients who are with cardio-vesicular deceases.
From this study, it indicates montmorillonite is a safe drug of carrier and no toxicity itself not only in vitro but also in vivo tests. And, montmorillonite does not has any adverse effects on gestation rats and embryos; I infer that montmorillonite may become a potent and safe drug of carrier in medicine in the future. However, it is still indefinite about montmorillonite for realistic application in replacement of chemical pesticides in agriculture. Therefore, I plan to farm tests to spray the optimum formula of montmorillonite-K6Fe(CN) 3-sodium alginate onto Chinese yam to further investigate if it keeps blades verdant on the Chinese yam owing to its bacteriostasis.
Subjects
蒙脫石
胚胎形成
受精卵著床
蒙脫石/海藻酸鈉複合物
麵包酵母
72小時急性毒性測試
Rats
72 hours acute toxicological tests
Hematology, Biochemistry
Zygocyte implant
Bacteriostasis
Dose-response analysis
embryo formation
Saccharomyces cerevisiae
montmorillonite
Type
thesis
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