Establishment and characterization of a human-papillomavirus negative, p53-mutation negative human cervical cancer cell line
Journal
Cancer Letters
Journal Volume
102
Journal Issue
1月2日
Pages
173-181
Date Issued
1996
Author(s)
Abstract
A human cervical cancer cell line, CX, was established from a patient with squamous cell carcinoma of the uterine cervix. The CX cells were epithelial in morphology with relatively large vesicular nuclei, and prominent nucleoli. Cytoplasmic organelles were generally sparse but tonofilaments were relatively abundant. The cells grew as a compact sheet with close membrane approximation interconnected by desmosome-like junctions. CX cells contained cytokeratin, but not vimentin. Elevated levels of squamous cell carcinoma antigen and carcinoembryonic antigen were detected in the cell supernatants. Population doubling time was estimated to be about 20 h. CX cells were not able to grow in soft agar and not tumorigenic in nude mice. Chromosome analysis revealed that CX cells were heterogeneous and mainly had a female diploid karyotype. Unlike cervical cancer cell lines published previously, CX cells were demonstrated to be human papillomavirus-negative, p53 mutation-negative, Based on the distinct characteristics, CX cell line may prove to be a useful tool for the study of human cervical carcinogenesis.
Subjects
Carcinogenesis; Cell line; Cervical cancer; Human papilloma virus; p53
SDGs
Other Subjects
cytokeratin; protein p53; article; cancer cell culture; cell nucleus; chromosome analysis; cytoplasm; desmosome; diploidy; human; human cell; karyotype; membrane vesicle; nonhuman; priority journal; squamous cell carcinoma; tonofilament; tumor suppressor gene; uterine cervix cancer; Wart virus; Human papilloma virus; Human papillomavirus; Human papillomavirus types; Mus musculus
Type
journal article