Detection of deformed wing virus (DWV) in the Vietnamese walking stick Medauroidea extradentata (Phasmatodea)
Journal
Virus Research
Journal Volume
293
Date Issued
2021
Author(s)
Abstract
Deformed wing virus (DWV) is a single-stranded positive sense RNA virus that mainly infects honey bees (Apis mellifera) and can have devastating impacts on the colony. Recent studies have shown the presence of this virus in several species of Apis spp. and some other Hymenoptera, but our knowledge of their host range is very limited. We screened previously sequenced RNAseq libraries from different tissues of Vietnamese Walking Stick, Medauroidea extradentata (Phasmatodea) for DWV. We only found this virus in six libraries from anterior and posterior midgut tissue. From the midgut libraries we were able to construct a complete DWV genome sequence, which consisted of 10,140 nucleotides and included one open reading frame. Pairwise genome comparison confirmed strong similarity (98.89 %) of these assembled sequences with only 113 SNPs to the original DWV genome. We hypothesize the M. extradentata acquired this virus via a foodborne transmission by consuming DWV-infected material such as pollen or leaves contaminated with virus infected bee faeces. ? 2020 Elsevier B.V.
Subjects
contig; nucleotide; polyprotein; transcriptome; virus DNA; amino acid sequence; animal tissue; Apis mellifera; Article; deformed wing virus; genomics; Medauroidea extradentata; midgut; nonhuman; nucleotide sequence; open reading frame; Phasmatodea; Picornavirales; priority journal; RNA sequencing; transcriptomics; Varroa destructor; Viet Nam; virus detection; virus genome; virus transmission
Type
journal article