Induction of cytochromes P450 1A1 and 1B1 in human lung adenocarcinoma CL5 cells by frying-meat emission particulate
Journal
Food and Chemical Toxicology
Journal Volume
40
Journal Issue
5
Pages
653-661
Date Issued
2002
Author(s)
Abstract
The effect of airborne frying-meat emission particulate (FMEP) on cytochrome P450 (P450)-dependent monooxygenase was determined using human lung adenocarcinoma cell line CL5 treated with organic extract of FMEP prepared from beef, fish or pork. Treatment with fish FMEP extract caused greater increases of intracellular peroxide production and glutathione content than did beef and pork FMEP extracts. Treatment with 200 μg/ml beef, fish or pork FMEP extract for 6 h increased benzo[a]pyrene hydroxylase, 7-ethoxyresorufin and methoxyresorufin O-dealkylases activities in S9. Immunoblot analysis of S9 proteins from control cells and cells treated with FMEP extracts revealed that the airborne particulates increased proteins immunorelated to CYP1A1 and CYP1B1. Northern blot analysis of total cellular RNA from controls and cells treated with FMEP extracts showed that the cooking by-products increased the levels of CYP1A1 and CYP1B1 mRNA. Treatment with 1 μM dibenzo[a,h]anthracene for 6 h increased monooxygenase activities, CYP1A1 and CYP1B1 protein and mRNA levels in CL5 cells. Beef FMEP extract and dibenzo[a,h]anthracene also induced CYP1A1 and CYP1B1 in human lung carcinoma NCI-H322 cells. The present finding demonstrates that airborne particulates generated during the frying of beef, fish and pork can induce carcinogen-metabolizing CYP1A1 and CYP1B1 in the human lung-derived cell line CL5. ? 2002 Elsevier Science Ltd. All rights reserved.
Subjects
CYP1A1; CYP1B1; Frying-meat emission particulate; Induction
Other Subjects
carcinogen; cytochrome P450; cytochrome P450 1A1; cytochrome P450 1B1; dibenz[a,h]anthracene; ethoxyresorufin; glutathione; messenger RNA; peroxide; unspecific monooxygenase; article; beef cattle; cancer cell culture; carcinogenesis; cell line; controlled study; cooking; enzyme activity; female; fish; frying; human; human cell; immunoblotting; lung adenocarcinoma; Northern blotting; Adenocarcinoma; Animals; Aryl Hydrocarbon Hydroxylases; Benz(a)Anthracenes; Blotting, Northern; Carcinogens; Cattle; Cell Line; Cell Survival; Cookery; Cytochrome P-450 CYP1A1; Cytochrome P-450 Enzyme System; Electrophoresis, Polyacrylamide Gel; Environmental Exposure; Fishes; Gas Chromatography-Mass Spectrometry; Humans; Lung Neoplasms; Meat; RNA, Messenger; Subcellular Fractions; Swine; Tumor Cells, Cultured
Type
journal article