在豬隻心房顫動模式中功能性基因體的變化(3/3)
Date Issued
2005
Date
2005
Author(s)
賴凌平
DOI
932314B002014
Abstract
Introduction Functional and structural changes of the atrial tissue occur during the
natural course of Af and these changes may contribute to further Af. We investigated
the changes in atrial fibrillation (Af) tissue using cDNA microarray and
two-dimensional protein electrophoresis techniques.
Methods and Results We established a porcine model of Af by rapid right atrial
appendage pacing at a rate of 600/min. Atrial tissue was obtained after rapid atrial
depolarization for 6 weeks. Microarrays containing 6035 cDNA clones were used to
evaluate the alterations of mRNA while two-dimensional protein electrophoresis was
performed to compare protein patterns. In cDNA microarray studies, we identified
387 genes with significant change in the left atrium and 81 genes in the right atrium.
Among the genes, the ventricular isoform of the myosin regulatory light chain
(MLC-2V) showed the greatest fold of change (9.4 and 7.3 in the left and right atrium
respectively). In protein electrophoresis, the expression levels of three protein spots
spanning from 18 to 20 Kd in the acidic region (PI 4.5 to 5.0) were specifically
elevated in the Af group. Interestingly, through tandem mass spectrometric analysis,
these three spots were identified to be MLC-2V. Thus MLC-2V expression at the
mRNA and protein levels corresponded well and both indicated a significant increase
in Af.
Conclusions Both cDNA microarray and 2D-PAGE studies revealed characteristic changes in Af tissue. We demonstrated the re-programming of myosin regulatory light
chain isoform composition with a significant increase of its ventricular isoform
(MLC-2V).
Subjects
atrial fibrillation
cDNA microarray
2-D PAGE
mass spectrometry
myosin regulatory light chain
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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