Regulation of Extracellular Matrix Metalloproteinase-3 （MMP-3）Promoter in MG-63 cells under Cyclic Tension and Compression Force Stimulation
Date Issued
2007
Date
2007
Author(s)
Tseng, Kuo-Jung
DOI
zh-TW
Abstract
It is widely accepted that mechanical loading is necessary to construct the architecture of bone and to maintain bone mass. Mechanical strain plays an important role in bone remodeling during growth and development. However, the cellular gene expression that controls the tissue response to mechanical force remains elusive. Previous experiments detected increased expression of matrix metalloproteinase-3 (mmp-3) when cyclic compression force was applied to MG-63 osteoblast-like cell in 3D collagen gel. Here, I cloned a 3.2 K-mmp-3 promoter fragment from human normal and HeLa cell by polymerase chain reaction and inserted this DNA into pGL3-Basic and pEGFP-1 vectors. Purified promoter-reporter constructs were transfected into MG-63 cells and cells were then processed through cyclic tension and compression stimulation for one day. The results indicate that the MMP-3 promoter activity was slightly down regulated by cyclic tension stimulation. However, promoter activity was up regulated by cyclic compression. Hence, mmp-3 promoter activity could be differentially regulated when osteoblasts experience different patterns of mechanical stimulation.
Subjects
週期性張力
週期性壓力
細胞外基質金屬蛋白
Cyclic Tension
Cyclic Compression
MMP-3 promoter
MG-63 cells
Type
other
File(s)![Thumbnail Image]()
Loading...
Name
ntu-96-R94450009-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):e8fdcf170d6dfcc5492c04f78d38e049