Toxin gene determination and evolution in scorpaenoid fish
Resource
Toxicon, 88, 21-33
Journal
Toxicon
Journal Volume
88
Pages
21-33
Date Issued
2014
Author(s)
Chuang, Po-Shun
Abstract
In this study, we determine the toxin genes from both cDNA and genomic DNA of four scorpaenoid fish and reconstruct their evolutionary relationship. The deduced protein sequences of the two toxin subunits in Sebastapistes strongia, Scorpaenopsis oxycephala, and Sebastiscus marmoratus are about 700 amino acid, similar to the sizes of the stonefish (Synanceia horrida, and Synanceia verrucosa) and lionfish (Pterois antennata and Pterois volitans) toxins previously published. The intron positions are highly conserved among these species, which indicate the applicability of gene finding by using genomic DNA template. The phylogenetic analysis shows that the two toxin subunits were duplicated prior to the speciation of Scorpaenoidei. The precedence of the gene duplication over speciation indicates that the toxin genes may be common to the whole family of Scorpaeniform. Furthermore, one additional toxin gene has been determined in the genomic DNA of Dendrochirus zebra. The phylogenetic analysis suggests that an additional gene duplication occurred before the speciation of the lionfish (Pteroinae) and a pseudogene may be generally present in the lineage of lionfish. © 2014 Elsevier Ltd. All rights reserved.
SDGs
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
Toxin gene determination and evolution in scorpaenoid fish.pdf
Size
1.95 MB
Format
Adobe PDF
Checksum
(MD5):36f4fe649b0b001f8af20ca4a28e7304