Gene Transfer in The Porcine and mouse Embryos
Date Issued
1998
Date
1998
Author(s)
鄭登貴
DOI
872313B002078
Abstract
The purpose of this study was to generate
transgenic mice and piglets by method of
microinjection the αLA- hFIX gene, a 4.93
kb fragment contained the regulatory
sequences of bovineα-lactoalbumin and the
structure sequence of cDNA from human factor
IX, into the pronucleus of newly fertilized
mouse and porcine eggs, respectively.
Experimental results showed eleven (2♀ / 9♂)
newborn mice and two (1♀ /1♂) of the
newborn piglets were confirmed to be as
transgenics, according the results from PCR
and Southern-blot analysis which had been
subjected to PCR and Southern-blot analysis
were comfirmed to be successfully integrated
with the transgene. The transgenic founders
mated with nontransgenic mice and pigs,
respectively, found the transgene stably
integrated in the germ line. Detecting by
Western-blot analysis confirmed that
expression of αLA-hFIX transgene with hFIX
concentration between 50-200μg/ml and 200-
500μg/ml contained in the milk from lactating
transgenic mice and sow, respectively. The
hFIX APTT clotting activity was assayed
directly on milk collected from transgenic mice
and pig, the data showed that the hFIX
synthesized by transgenic mice and pig is
active. Furthermore, frozen semen of L-8-5
were prepared and inseminated to
nontransgenic sow, could transmit the
transgene to progenies, demonstrating that the
transgenic line can be preserved by frozen
semen. In the future, focusing our attention on
economic consequences and product safety
may be important.
Publisher
臺北市：國立臺灣大學動物科學技術學系暨研究所
Type
report
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