Monodansylpentane as a Blue-Fluorescent Lipid-Droplet Marker for Multi-Color Live-Cell Imaging
Resource
PLOS ONE, 7(3), e32693
Journal
PLoS ONE
Pages
e32693
Date Issued
2012
Date
2012
Author(s)
Yang, Huei-Jiun
Hsu, Chia-Ling
Yang, Jin-Yi
Yang, Wei Yuan
Hong, Wanjin
Abstract
Lipid droplets (LDs) are dynamic cellular organelles responsible for the storage of neutral lipids, and are associated with a multitude of metabolic syndromes. Here we report monodansylpentane (MDH) as a high contrast blue-fluorescent marker for LDs. The unique spectral properties make MDH easily combinable with other green and red fluorescent reporters for multicolor fluorescence imaging. MDH staining does not apparently affect LD trafficking, and the dye is extraordinarily photo-stable. Taken together MDH represents a reliable tool to use for the investigation of dynamic LD regulation within living cells using fluorescence microscopy.
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
20.pdf
Size
23.23 KB
Format
Adobe PDF
Checksum
(MD5):624ebbee7801491bde2a8dcb6a51e897