Genetic Uniformity within Rice Cultivars Measured by SSR Markers
Date Issued
2004
Date
2004
Author(s)
Wu, Dai-Rong
DOI
zh-TW
Abstract
The within-cultivar uniformity of six rice cultivar has been investigated using 20 SSR markers with fluorescence-based automated DNA detection technology in this study. In all 22 loci detected, 18 and 11 loci are polymorphic between and within cultivar, respectively. There are two type of non-uniformity within cultivars, the overwhelming one is the type that has more than one homozygotes in a locus, the other one is hetetozygote. According to the result of principle coordinate analysis, cluster analysis and Assignment test, the cultivars were uniquely identifiable in spite of different level of existing within-cultivar non-uniformity. To discuss the boundary of distinctness between cultivars and uniformity within cultivars, genetic distance and Assignment test were used, and the result revealed genetic information of most samples fall within the standards. The within-cultivar non-uniformity may come from mutation, residual heterozygosity, the seed purity of rice sample used, open-pollination rate and experimental error, which includes the number of cultivars used and number of plants within cultivars sampled.
Subjects
分子標記
均一性
品種內
within cultivar
uniformity
SSR
Type
thesis
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ntu-93-R91621106-1.pdf
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