Structure and biological function of mmuno-modulating molecules of wheat grass and anoderma lucidum polysaccharides
Date Issued
2009
Date
2009
Author(s)
Tsai, Chia-Che
Abstract
Natural products have played an important role throughout the world in preventing and treating human diseases throughout the history in different parts of the world. The Monocotyledoneae plant wheat grass and the Ganoderma lucidum polysaccharides were served as materials in this study to identify the molecules with immuno-modulating properties. By cytomic screening, the immuno-modulating behavior of the bioactive oligosaccharides (WG-PS3) from wheat grass has been described. WG-PS3 activated monocytes, T cells, and NK cells in human peripheral blood mononuclear cell (hPB-MNC). WG-PS3 was observed directly activate the purified monocytes by inducing the expression of CD69, CD80, CD86, IL-12, and TNF-a. In contrast, WG-PS3 indirectly affected NK and T cells only in the presence of monocytes. The structure of WG-PS3 was characterized suggesting repeating (a,b)-1,4-hexose. After HPLC separation, a hepta-1,4-glucan were identified from WG-PS3 and exhibited significantly ability to induce monocytes activation. anoderma lucidum polysaccharides F3 were macromolecules with molecular weight around 500KD. F3 was found that processed the ability to directly induce NK cells, T cell, and monocytes activation by cytomic screening. By Smith degradation, we successfully degraded and fractionalized the F3. All of these fractions retained the CD69 induction activity to monocytes, T cells, and NK cells in hPB-MNC. However, a fraction F3S-G3 with structural signature of b-(1,4)-Glucorounic acid / Glucose oligomer was presented the specific immune property of low monocyte induction activity and high ability to stimulate IL-2 secretion in NK and T cells. In addition, the fraction F3S-G4 with low molecular weight around 1000 Dalton retained all the immuno-activating characteristics of F3 that described in above cytomic screening. The F3S-G4 involved heteroglycan and could be further separated by HPLC. Thus, the F3S-G4 may serve the materials for further analysis of defined structure.
Subjects
wheat grass
Ganoderma lucidum polysaccharides
oligosaccharides
immuno-modulation
mononuclear cells
SDGs
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