鯉魚視網膜桿狀細胞受光蛋白基因的調控
Date Issued
1999-07-31
Date
1999-07-31
Author(s)
蔡懷楨
DOI
882311B002046
Abstract
Rod opsin, rhodopsin, is one of the photoreceptors but locates at rod cells, which
mediates dim-light vision. We have cloned rhodopsin gene from the common carp
( Cyprinus carpio). The deduced amino acid sequence of carp rhodopsin showed 95.7, 85.5
and 74.4 % identity with that of goldfish, sand goby and lamprey, respectively. However,
few significant homological regions could be found in the 5’ regulatory region compared
with corresponding sequences of other terrestrial species. To define the cis-acting DNA
elements required for rhodopsin expression, we generated transgenic fish carrying
sequences upstream of carp rhodopsin gene fused to the green fluorescence protein cDNA
as a reporter gene. Upstream sequences extending from –6000 to +66 bp and from –146 to
+94 bp were able to drive retinal-specific expression, whereas from –53 to +94 bp segment
was not. This suggests that element which regulates retinal-specific expression may locate
within –146~ -53 bp. Interestingly, little homology of polynucleotide sequences was shown
between the region (-146~ -53 bp) and any other known retina-specific protein binding sites
of terrestrial animals.
Publisher
臺北市：國立臺灣大學漁業科學研究所
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
882311B002046.pdf
Size
209.19 KB
Format
Adobe PDF
Checksum
(MD5):13bdf0b4b094d97bf19788be8fd2c52b