Single fluorescence imaging to observe the dipole radiation appearance between two same dye
Date Issued
2008
Date
2008
Author(s)
Yu, Min-Long
Abstract
From 2000，Jörg Enderlein had some articles about experiment and theory in single molecular Cy5 defocus image in a sensitive CCD camera. Single fluorescence imaging method for direct determination of single-molecule orientations is presented that uses a wide-field epifluorescence microscope and a sensitive CCD camera. Jörg Enderlein had good results in theory and experiment. In this thesis，we use his result to observe the dipole radiation appearance between two same dye. In single molecule mage method , two dye’s dipole have different orientation to show different defocus pattern in CCD camera and pattern’s relative distribution. In Molecular Dynamic simulation，we use “Gromacs Package” to simulate the equilibrium structure of DNA which is labeled TMR in solution in water solution. We want to find the local minimus energy ,and MM_PBSA model’s free energy. Double strand linear DNA is the sample to do defocus image . We want to use DNA to control the distance and angle between two dye.But the condition in experiment is not solution. So, result of MD part is reference.
Subjects
single molecular
wide field
defocus
phase
Type
thesis
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