Monitoring Microorganisms in Hot Spring Water by Fluorochrome Method
Date Issued
2005
Date
2005
Author(s)
Chen, Chia-Li
DOI
zh-TW
Abstract
Total concentration, viability, and culturability of microorganisms in 48 hot spring water samples were monitored by using epifluorescence microscopy and flow cytometry with fluorochrome (EFM/FL) with five fluorescent dyes (AO, DAPI, SYTO-13 PI, and YOPRO-1). Results from the non-culture-based method were then compared with those using a commonly used culture method. The total cell concentrations measured using the non-culture-based EFM/FL methods were from 1.08x105 to 6.16x106cells/ml with AO staining, and from 1.14x105 to 6.80x106 cells/ml with DAPI staining. And the total cell concentrations measured using the non-culture-based FCM/FL methods were from 1.30x105 to 1.04x107cells/ml with AO staining, and from 2.51x105 to 1.07x107cells/ml cells/ml with SYTO-13 staining. The Results compared with the concentration range of 0 to 4.60x104 CFU/ml for bacteria and 0 to 1.64x103 CFU/ml for fungi by the culture method. The viability ranged from 0 to 0.76 with PI staining, from 0 to 0.63 with YOPRO-1 staining, and from 0 to 0.082 by the culture method. The viability by EFM/FL was much higher than the culturability.
Our results clearly show that the traditional culture method underestimates the total concentration and viability of microorganisms in hot spring water. Therefore, to reduce health risks, a comprehensive microorganism monitoring in hot spring water is needed to provide more accurate and representative information. In our present study, EFM/FL and FCM/FL technique was successfully applied to quantify the total concentration and viability of microorganisms in hot spring water, and can be used for different environmental environments.
Subjects
溫泉
螢光染色法
螢光顯微鏡
流式細胞儀
hot spring
fluorochrome method
epifluorescence microscopy
flow cytometry
SDGs
Type
thesis