Reconstitution of Severe Combined Immunodeficient Mice with Spleen Cells from Autoimmune Nzbxnzw F1 Mice
Resource
CLINICAL AND EXPERIMENTAL RHEUMATOLOGY v.16 n.1 pp.33-37
Journal
CLINICAL AND EXPERIMENTAL RHEUMATOLOGY
Journal Volume
v.16
Journal Issue
n.1
Pages
33-37
Date Issued
1998
Date
1998
Author(s)
CHIANG, BOR-LUEN
Abstract
Objective:The aim of this study is to establish an animal model to investigate the role of individual subsets of immune cells in the pathogenesis of systemic lupus erythematosus.Methods:Spleen cells isolated from both young and old autoimmune NZB/W F1 mice were injected into the peritoneal cavity of severe combined immunodeficient (SCID) mice. Sera anti-DNA antibody levels and proteinuria of these SCID mice were followed regularly. In addition, histological changes of the kidneys were also examined. Results:The data suggest that anti-ss, dsDNA antibody can be detected in the sera of SCID mice 21 days after reconstitution with the spleen cells of either young or old NZB/W Fl mice, with titers of antibody increasing over time. In addition, proteinuria was also noted in most of these mice 3 months after reconstitution. Histopathological examination of the kidney also revealed the typical changes of glomerulonephritis. Immunofluorescence staining of kidney sections also demonstrated immune complex deposition. Conclusion:Once validated, this animal model could be used in future studies to investigate the role of individual subsets of cells in the pathogenic mechanisms of SLE.
Type
journal article