Allicin induces anti-human liver cancer cells through the p53 gene modulating apoptosis and autophagy
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
61
Journal Issue
41
Pages
9839-9848
Date Issued
2013
Author(s)
Abstract
Hepatocellular carcinoma (HCC) is the most prevalent type of liver cancer globally and ranks first among the cancer-related mortalities in Taiwan. This study aims to understand the modes of cell death mechanism induced by allicin, a major phytochemical of crushed garlic, in human hepatoma cells. Our earlier study indicated that allicin induced autophagic cell death in human HCC Hep G2 (p53wild type) cells, whereas in the present study, allicin induced apoptotic cell death through caspase-dependent and caspase-independent pathways by reactive oxygen species (ROS) overproduction in human HCC Hep 3B (p53 mutation) cells. To gain insight into the cell death mechanism in p53 knocked down Hep G2, we silenced the p53 gene using siRNA-mediated silencing. Allicin treatment induced apoptotic cell death in p53 knocked down Hep G2 cells similar to that of Hep 3B cells. These results suggest that allicin induced cell death in human hepatoma cells through either autophagy or apoptosis and might be a potential novel complementary gene therapeutic agent for the treatment of apoptosis-resistant cancer cells. ? 2013 American Chemical Society.
Subjects
Allicin
apoptosis
autophagy
p53
reactive oxygen species
SDGs
Other Subjects
Allicin; Apoptotic cell death; Autophagy; Gene therapeutic agents; Hepatocellular carcinoma; Human hepatoma cells; p53; Reactive oxygen species; Diseases; Gene therapy; Oxygen; Tumors; Cell death; allicin; plant extract; protein p53; sulfinic acid derivative; TP53 protein, human; apoptosis; article; autophagy; cell strain HepG2; chemistry; drug effect; garlic; genetics; human; liver tumor; metabolism; pathophysiology; Apoptosis; Autophagy; Garlic; Hep G2 Cells; Humans; Liver Neoplasms; Plant Extracts; Sulfinic Acids; Tumor Suppressor Protein p53
Type
journal article