Functional Roles of PARP7 Oral Cancer Cells
Date Issued
2008
Date
2008
Author(s)
Lin, Pei-Yu
Abstract
There are approximately 2 million habitual chewers in Taiwan. According to various studies, it has been considered that betel quid chewing to be an independent risk factor in the development of oral cancers . As a result, the International Agency for Research on Cancer (IARC) has evaluated Areca nut (AN), the main component in various forms of betel preparations, as a carcinogen to human in 2003. However, the definite pathogenesis and multifactorial mechanisms implicated by AN chewing are still not fully understood.recoline is the main alkaloid of AN with a soluble feature in water and alcohol. This compound is mutagenic and increases the risk of chromosome instability. Poly (ADP-ribose) polymerases (PARPs) comprise a protein family involved in a number of cellular processes including DNA repair and programmed cell death. Its functions have been correlated to cancer formation from several aspects and recently the use of PARP inhibitors as anti-cancer therapy becomes longstanding approach from different laboratories in last decades. In this study, we analyzed the expression patterns of PARPs in arecoline induced SAS oral cancer cells. We found that PARP7, as a member of PARPs, is up-regulated in in this condition in both mRNA and protein level. Using immuno-fluorescence staining, we found that exogenous PARP7 locate at the cell nucleus. Furthermore, we demonstrated that the product of PARP7 catalytic domain has ADP-ribosylation activity. Our data reveal that PARP7 is not a conventional PARP, which add long chain of ADP-ribose moieties to acceptor proteins. In contrast, it adds one or less than 6 ADP-ribose to histones. Finally, we analyzed that PARP7 associates with NBS1, one of the key members participating in homologous recombination by protein mass spectrometry. Together, our results indicate that PARP7 may be involved in DNA repair which is induced by arecoline treatment. We anticipate that our results can provide a reference for future drug development for oral cancer therapy.
Subjects
Function
Oral Cancer
SDGs
File(s)![Thumbnail Image]()
Loading...
Name
ntu-97-R95450004-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):295acdbe0c31401b48cb598cde22c056