Silica nanoparticles for separation of biologically active amines by capillary electrophoresis with laser-induced native fluorescence detection
Resource
Electrophoresis 26 (13): 2643-2651
Journal
Electrophoresis
Journal Volume
26
Journal Issue
13
Pages
2643-2651
Date Issued
2005
Author(s)
Abstract
This paper describes the analysis of biologically active amines by capillary electrophoresis (CE) in conjunction with laser-induced native fluorescence detection. In order to simultaneously analyze amines and acids as well as to achieve high sensitivity, 10 mM formic acid solutions (pH < 4.0) containing silica nanoparticles (SiNPs) were chosen as the background electrolytes. With increasing SiNP concentration, the migration times for seven analytes decrease as a result of increase in electroosmotic flow (EOF) and decrease in their electrophoretic mobilities against EOF. A small EOF generated at pH 3.0 reveals adsorption of SiNPs on the deactivated capillary wall. The decreases in electrophoretic mobilities with increasing SiNP concentration up to 0.3 x indicate the interactions between the analytes and the SiNPs. Having a great sensitivity (the limits of detection at a signal-to-noise ratio (S/N) = 3 of 0.09 nM for tryptamine (TA)), high efficiency, and excellent reproducibility (less than 2.4% of the migration times), this developed method has been applied to the analysis of urinal samples with the concentrations of 0.50 ± 0.02 μM, 0.49 ± 0.04 μM, and 74 ± 2 μM for TA, 5-hydroxytryptamine, and tryptophan, respectively. The successful examples demonstrated in this study open up a possibility of using functional nanoparticles for the separation of different analytes by CE. ? 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Subjects
Capillary electrophoresis laser-induced native fluorescence; Catecholamines; Silica nanoparticles
Other Subjects
1 naphthol; 1 naphthylacetic acid; adrenalin; amine; dopamine; electrolyte; formic acid; nanoparticle; serotonin; silicon dioxide; tryptamine; tryptophan; article; capillary electrophoresis; chemical analysis; chemical interaction; chemical structure; concentration (parameters); controlled study; diagnostic value; electroosmosis; electrophoretic mobility; female; flow kinetics; fluorescence analysis; human; laser; neurologic disease; normal human; pH; reproducibility; sensitivity analysis; separation technique; signal noise ratio; time; urinalysis; Body Fluids; Catecholamines; Electrophoresis, Capillary; Female; Humans; Lasers; Nanostructures; Reproducibility of Results; Sensitivity and Specificity; Silicon Dioxide; Spectrometry, Fluorescence
Type
journal article
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