Plant regeneration from protocorm-derived callus of Cypripedium formosanum
Journal
In Vitro Cellular and Developmental Biology - Plant
Journal Volume
39
Journal Issue
5
Pages
475-479
Date Issued
2003
Author(s)
Lee, N.
Abstract
Totipotent callus of Cypripedium formosanum, an endangered slipper orchid species, was induced from seed-derived protocorm segments on a quarter-strength Murashige and Skoog medium containing 4.52 μM 2,4-dichlorophenoxyacetic acid and 4.54 μM 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (thidiazuron). This callus proliferated well and was maintained by subculturing on the same medium. On average, 13 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the medium with 4.44 μM N 6-benzyladenine after 8 wk of culture. The regenerated protocorm-like bodies formed shoots and roots on medium containing 1 gl -1 activated charcoal and 20 gl-1 potato homogenate. After 24 wk of culture on this medium, well-developed plantlets ready for potting were established.
SDGs
Publisher
BioOne
Type
journal article