Irreversible cellular senescence induced by H2O2 treatment of cells cultivated on the PVA membrane
Date Issued
2009
Date
2009
Author(s)
Chen, Yi-Shun
Abstract
Introduction: It has been known that different chemical and physical surface properties of biomembranes controlled the cell behavior, such as cell survival and growth. However, there is no report which is focused on the effects of biomembranes to cellular senescence. Thus, the objective of this study was to investigate the effects of cell aggregates cultivated on the surface of PVA biomembranes. The influences of biomembranes to the proliferation and senescence of cells were also studied.aterials and methods: We chose three kinds of biomembranes, PVA (polyvinyl Alcohol) BP-20H has high alkalization degree, PVA (polyvinyl Alcohol) BC-24 has high viscosity, and tissue culture polystyrene (TCPS). The survival and proliferation of H1299 and H1299/FAK when cultured on PVA membranes were investigated. Human foreskin fibroblast (HFF) were cultured on these membranes and received different conditions of H2O2 treatment to establish senescence correlation index, such as growth curves (P.D) and SA-β-galactosidase activity. Senescence associated gene expression was also analyzed.esults: Our results show that H1299/FAK has better survival and WST-1 value than the H1299. Prolonged low-dose H2O2 treatment not only induced irreversible cell growth arrest but also increased senescent cells, especially cultured on the PVA membranes.onclusions: H1299/FAK has better survival on the PVA membranes than the H1299 cells. H2O2 treatment significantly induced irreversible cell growth arrest and the percentage of senescent cells to the HFF cells. This phenomenon could be further enhanced by transferring the H2O2 treated cells to the PVA membranes for subsequent cell culture.
Subjects
Polymer biomembranes
PVA (polyvinyl Alcohol)
fibroblast
Cellular senescence
Type
thesis
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