Analysis the Cis-elements of Mungbean Rubisco Activase Promoter under Chilling Stress
Date Issued
2005
Date
2005
Author(s)
許文賢
DOI
zh-TW
Abstract
The mungbean (Vigna radiata L. v. 2937) is a chilling-sensitive plant. A cDNA library was constructed from poly(A)+ mRNA of 28℃-light-grown leaves for screening cold-suppressed nuclear genes. Rubisco activase(rca) gene was one of the 13 cold-suppressed nuclear genes which obtained by differential screening. Rubisco activase gene has single copy in mungbean (Vigna radiata L. v. 2937) and is light-inducible. Under 10℃ chilling stress, rca mRNA synthesis was repressed and circadian rhythm was interrupted. The upstream region (1,161bp) of rca gene had been cloned from mungbean by genomic walking method. The results of promoter deletion assay, Arabidopsis transformation and gel retardation have defined the promoter region from -521 to -431 which was related to the chilling suppression and also bound by nuclear proteins. Those nuclear proteins were isolated from cold-treated (3 days, 10℃) mungbeans. Using the PLACE website, the promoter region from -521 to -431 has a specific region(DOFCOREZM). In other study, the Dof2 protein of maize bound DOFCOREZM region and suppressed the activity of the C4pepc promoter (Yanagisawa, 2000). Dof analogies of mungbean maybe suppress rca gene expression under chilling stress.
Subjects
低溫逆境
綠豆
Chilling Stress
Mungbean
Rubisco Activase
Type
other
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