NF-kB雙股寡核酸抑制動情素接受體陰性乳癌細胞株生長及轉移之研究
The Effect of NF-kB Decoy on MDA-MB-231 Breast Cancer Cell Line: Inhibition of Cell Growth and Metastasis.
Date Issued
2004
Date
2004
Author(s)
An, Feng-Ming
DOI
en-US
Abstract
Numerous experimental data and clinical studies have established that estrogen plays a major role in the initiation and progression of breast cancers. Thus antiestrogens were used but did not work on estrogen receptor (ER)-negative breast cancer cells. Another therapeutic target we are interested in is NF-kB. NF-kB was expressed constitutively in ER-negative breast cancer cells and might affect cell growth and metastasis. Thus inhibition of NF-kB is a plausible way to develop anti-cancer drugs.
Therefore, our strategy is using synthetic oligonucleotides containing NF-kB binding sequence, which is 21 bps in length and is modified at both ends with phosphorothioate linkages. This NF-kB decoy demonstrates NF-kB binding activity and can be easily measured by electrophoresis mobility shift assay (EMSA). Our data showed that transfection of NF-kB decoy to ER-negative breast cancer cell line, MDA-MB-231, significantly block NF-kB activation. To confirm the transfection efficiency, we used fluorescein-labeled NF-kB decoy (F-decoy) and analyzed by flow cytometry and fluorescence microscopy. For cell viability, we used ACP assay to measure the living cells after treating NF-kB decoy or not. Our results showed that relatively high concentration was needed to cause significant inhibition of cell growth than inhibition of cell migration. On the other hand, it is found that NF-kB decoy significantly inhibits adhesion and migration of MDA-MB-231 cells. Whereas NF-kB cause widely effects on cell adhesion, invasion, and migration, we focused on the interaction of CXCR4 and SDF-1
Subjects
乳癌
雙股寡核酸
轉移
Metastasis
Breast Cancer
Decoy
SDGs
Type
other
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