Wound-response regulation of the sweet potato sporamin gene promoter region
Resource
Plant Molecular Biology 48 (3): 223-231
Journal
Plant Molecular Biology
Journal Volume
48
Journal Issue
3
Pages
223-231
Date Issued
2002
Date
2002
Author(s)
Abstract
Sporamin, a tuberous storage protein of sweet potato, was systemically expressed in leaves and stems by wound stimulation. In an effort to demonstrate the regulatory mechanism of wound response on the sporamin gene, a 1.25 kb sporamin promoter was isolated for studying the wound-induced signal transduction. Two wound response-like elements, a G box-like element and a GCC core-like sequence were found in this promoter. A construct containing the sporamin promoter fused to a beta-glucuronidase (GUS) gene was transferred into tobacco plants by Agrobacterium-mediated transformation. The wound-induced high level of GUS activity was observed in stems and leaves of transgenic tobacco, but not in roots. This expression pattern was similar to that of the sporamin gene in sweet potatoes. Exogenous application of methyl jasmonate (MeJA) activated the sporamin promoter in leaves and stems of sweet potato and transgenic tobacco plants. A competitive inhibitor of ethylene (2,5-norbornadiene; NBD) down-regulated the effect of MeJA on sporamin gene expression. In contrast, salicylic acid (SA), an inhibitor of the octadecanoid pathway, strongly suppressed the sporamin promoter function that was stimulated by wound and MeJA treatments. In conclusion, wound-response expression of the sporamin gene in aerial parts of plants is regulated by the octadecanoid signal pathway.
SDGs
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
17.pdf
Size
213.1 KB
Format
Adobe PDF
Checksum
(MD5):0dbb6a32276bfd40d7a7720f815945ce