生體組織修復材料之研發─骨科複合材料-可吸收性生醫陶瓷管柱體內塞去礦化骨及生長淚素複合材料(2/3)
Date Issued
1999-07-31
Date
1999-07-31
Author(s)
林輝
DOI
882314B002040M08
Abstract
Autogenous bone transfer has been an important part of reconstructive plastic
surgery. Presently, available technique have the disvantages of limitation of availablee
donor site, loss amount of donor tissue, and possibility of donor defect or deformity.
In the present study, a vascularized bone graft was created and cultured in the groin
area of the New Zealand rabbit. The cylindrical ceramic chambers with 15 mm in
length, 6 mm in outer diameter, and 3 mm in inner diameter were prepared by the
sintered porous -Ca2P2O7 with 5 wt% Na4P2O7 10H2O addition. In the first
group, the chambers impregnated with autogenous bone fragments and allogenous
demineralized bone matrix with volum ratio 1:1 were cultured in the rabbit groin
area with saphenous vessels passing through. In the second group, the chambers were
treated as the same procedures as the first group without saphenous vessels passing
through. In the third group, the chambers impregnated nothing were also cultured in
the groin area with saphenous vessel passing through.
After 2, 4, 6, 8, and 12 weeks of operation, the animals were executed with an
overdose of intravenous pentobabital. The viability of the osseous tissue in the
chamber was evaluated by the histological examination, microangiograms, and
fluorochrome incoorperation for the three groups. The autogenous bone chips could
survive and retain their osteogenic properties while packed into the sintered porous
-Ca2P2O7 (with 5 wt% Na4P2O7 10H2O addition) ceramic chamber and
implanted into the rabbit groin area upto 12 weeks. However,even at the longest time
periods, considerable amounts of dead bone were present in the chambers. In addition,
we observed bone resorption in the three groups upto 12 weeks, which might be
attributed to lacking of physiological stress. There were significant difference in new
bone formation and osseous cells viability among the three groups. The
prevescularized vessels and autogenous bone chips were both necessary for the new
bone formation and osteogenic property in the chamber under such a heterotopic
circumstance. The biodegradable ceramic used in this study were gradually absorbed
and dissolved in the physiological environment. However, the degradation debris of
the ceramic showed no harm and no injury to the new bone formation. These findings
support the concept of creating a preformed vascularized bone graft to reconstruct
segmental bone defects.
Publisher
臺北市：國立臺灣大學醫學工程學研究所
Type
report
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