Non-Enzymatic and Electrodeless Detection of Direct Bilirubin Using Metal Enhanced Fluorescence Effect
Journal
Proceedings of the ASME Design Engineering Technical Conference
Journal Volume
1
ISBN
9780791883907
Date Issued
2020-01-01
Author(s)
Abstract
Copyright © 2020 ASME. In this study, a metal enhanced fluorescence (MEF) approach using the homemade Ag nanowires for enzyme free and electrodeless detection of direct bilirubin (d-BLR) was successfully developed. The average diameter and length of the fabricated Ag nanowires were measured to be around 80 nm and 25 ï?-m, respectively. Solvent effect experiments found that EtOH is more suitable as the solvent for d-BLR. The experimental results found that the linear detection range is from 1 to 10 ï?-M, which can be used for detecting abnormal d-BLR concentration from 5 to 10 ï?-M. The MEF approach not only enhanced the bilirubin fluorescence, but also improved the linearity of the standard curve. Furthermore, we also found that the fluorescence intensity of the chip-based MEF is much higher than that of the solution-based MEF. It is believed that the proposed enzyme free and electrodeless d-BLR detection platform is highly feasible for clinical applications.
Subjects
Ag nanowire | Direct bilirubin detection | Enzyme free and electrodeless detection | Metal enhanced fluorescence
Type
conference paper