Multiphoton fluorescence lifetime imaging microscopy (FLIM) of Bilirubin oxidants of oral cancer cells
Date Issued
2012
Date
2012
Author(s)
Huang, Cheng-Jie
Abstract
We have observed the red fluorescence of five oral cell lines with multi-photon fluorescence lifetime and intensity. With lifetime trace, single photon, and two photon fluorescence spectrum, we found out that biliverdin is one of red fluorescence source (exciting spectrum peak around ~660nm). Four oral cancer cells from tongue and a non-cancer oral cell lines: S-G (normal oral cell as control group), DOK (pre-cancer oral cell), OECM-1, SCC-15, and HSC-3(different stage of malignancy) were investigated by a femtosecond Cr:forsterite laser -based multi-photon fluorescence lifetime image. Due to shift of metabolic status, we used two component to fit lifetime histgrom and detected that DOK and OECM-1 has longer t1 lifetime(~500ps), SCC-15 and HSC-3 have relatively short 370ps, and normal oral, S-G cells have much shorter t1 lifetime(~270ps). In addition, S-G cell have visible shorter t2 lifetime (~1.6ns), cancer cells general longer (~1.9ns). With our study result, we believe this lifetime of biliverdin fluorescence is potential to serve as an diagnostic index on the stage and malignancy of oral cancers in the future.
Subjects
fluorescence
lifetime
oral cancer
bilirubin
FLIM
SDGs
Type
thesis
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