Metabolic Alterations in Shrimp Stomach During Acute Hepatopancreatic Necrosis Disease and Effects of Taurocholate on Vibrio parahaemolyticus
Journal
Frontiers in Microbiology
Journal Volume
12
Date Issued
2021
Author(s)
Abstract
Acute hepatopancreatic necrosis disease (AHPND), a recently emerged bacterial shrimp disease, has increased shrimp mortality and caused huge economic losses in many Asian countries. However, molecular factors underlying pathogenesis of this disease remain largely unknown. Our objective was to characterize metabolic alterations in shrimp stomach during AHPND and determine effects of taurocholate on AHPND-causing Vibrio parahaemolyticus. Based on metabolomics, pathways for lipid metabolism and for primary bile acid (BA) synthesis were majorly affected following AHPND infection. Bile acid metabolites, namely taurocholate, were downregulated in the metabolomics database. This prompted us to study effects of taurocholate on biofilm formation, PirABvp toxin release and biofilm detachment capabilities in AHPND-causing V. parahaemolyticus. Treatment of this bacterium with high concentration of taurocholate, a primary bile acid, induced biofilm formation, PirABvp toxin release and facilitated the dispersion of bacterial cells. Taken together, our findings suggest that AHPND infection can affect the lipid metabolites in shrimp stomach, and further suggest that the primary bile acid taurocholate is important for the virulence of AHPND-causing V. parahaemolyticus. ? Copyright ? 2021 Kumar, Tung, Ng, Chang, Chen, Chen, Lin and Wang.
Subjects
bile acid; luminol; oligosaccharide; racemase; taurocholic acid; acute hepatopancreatic necrosis disease; animal experiment; Article; bacterial virulence; bile acid synthesis; biofilm; diet supplementation; dispersion; gene expression; lipid metabolism; metabolomics; mortality; nonhuman; real time polymerase chain reaction; stomach; ultra performance liquid chromatography; Vibrio parahaemolyticus
SDGs
Type
journal article