Biocompatible transferrin-conjugated sodium hexametaphosphate-stabilized gold nanoparticles: synthesis, characterization, cytotoxicity and cellular uptake
Journal
Nanotechnology
Journal Volume
22
Journal Issue
39
Date Issued
2011
Author(s)
Parab, Harshala J.
Abstract
The feasibility of using gold nanoparticles (AuNPs) for biomedical applications has led to considerable interest in the development of novel synthetic protocols and surface modification strategies for AuNPs to produce biocompatible molecular probes. This investigation is, to our knowledge, the first to elucidate the synthesis and characterization of sodium hexametaphosphate (HMP)-stabilized gold nanoparticles (Au-HMP) in an aqueous medium. The role of HMP, a food additive, as a polymeric stabilizing and protecting agent for AuNPs is elucidated. The surface modification of Au-HMP nanoparticles was carried out using polyethylene glycol and transferrin to produce molecular probes for possible clinical applications. Invitro cell viability studies performed using as-synthesized Au-HMP nanoparticles and their surface-modified counterparts reveal the biocompatibility of the nanoparticles. The transferrin-conjugated nanoparticles have significantly higher cellular uptake in J5 cells (liver cancer cells) than control cells (oral mucosa fibroblast cells), as determined by inductively coupled plasma mass spectrometry. This study demonstrates the possibility of using an inexpensive and non-toxic food additive, HMP, as a stabilizer in the large-scale generation of biocompatible and monodispersed AuNPs, which may have future diagnostic and therapeutic applications. ? 2011 IOP Publishing Ltd.
SDGs
Other Subjects
Aqueous medium; Biomedical applications; Cell viability; Cellular uptake; Control-cell; Fibroblast cells; Gold nanoparticle; Gold Nanoparticles; In-vitro; Liver cancer cells; Molecular Probes; Mono-dispersed; Oral mucosa; Possible clinical applications; Protecting agent; Sodium hexametaphosphate; Surface-modified; Synthetic protocols; Therapeutic Application; Biocompatibility; Cell culture; Food additives; Glycols; Inductively coupled plasma; Inductively coupled plasma mass spectrometry; Medical applications; Nanoparticles; Polyethylene glycols; Probes; Sodium; Gold; biomaterial; gold; nanoparticle; phosphate; sodium polymetaphosphate; transferrin; article; cell line; cell membrane permeability; cell survival; chemistry; human; metabolism; surface property; ultrastructure; Biocompatible Materials; Cell Line; Cell Membrane Permeability; Cell Survival; Gold; Humans; Nanoparticles; Phosphates; Surface Properties; Transferrin
Type
journal article