A novel gene, BC1, contributes to oncogenic transformation in vitro and in vivo
Date Issued
2004
Date
2004
Author(s)
Chou, Fang-Yu
DOI
en-US
Abstract
We have isolated a novel gene, BC1, from the GCH (GTP cyclohydrogenase I) dominant negative cell models. BC1 is a 74KD polypeptide found in brain and also in 16 cell lines we tested. In the previous studies, BC1 could prevent Huntingtin induced aggregation as well as diminish cell toxicity.
Chromosome 20q13 has been identified as gene amplification in cancers such as ovarian, breast, colon and pancreatic carcinoma. BC1 resides in this region, thus, we are interested in (1) is BC1 oncogenic? (2) Whether the inhibition of BC1 reduces tumor progression?
In this study, we first established BC1 expression cell line and found that overexpression of BC1 exhibited significant advance of cell proliferation, induction transformation ability in soft agar assay, and stimulation of xenograft tumor growth in Nod-SCID mice.
Second, we generate BC1-knockdown cell lines by introducing BC1 antisense plasmid. Our results reveal that down-regulation of BC1 expression can effectively inhibit the proliferation of human breast cancer cells, and BC1 may serve as a drug target in carcinogenesis.
These results establish a role for BC1 in oncogenesis and cell survival and also demonstrate a potential strategy for cancer therapy.
Subjects
致癌基因
oncogenic transformation
SDGs
Type
other