Fibrin glue mixed with gelatin/hyaluronic acid/hondroitin-6-sulfate tri-copolymer for articular cartilage tissue engineering
Date Issued
2008
Date
2008
Author(s)
Chou, Cheng-Hung
Abstract
Articular cartilage provides functions of lubrication to shear stress and protection from compressive force, but it has poor ability to repair itself after suffering damage. The advanced method of tissue engineering is developed and used to maintain cell functions for tissue regeneration. Autologous fibrin glue has been demonstrated as a potential scaffold with very good biocompatibility for neocartilage formation. However, fibrin glue has been reported not to provide enough mechanical strength, but with many growth factors to interfere the tissue growth. Gelatin/hyaluronic acid/chondroitin-6-sulfate (GHC6S) tricopolymer sponge has been prepared as scaffold for cartilage tissue engineering and showed very good results, but problems of cell seeding and cell distribution troubled the researchers. In this study, GHC6S particles would be added into the fibrin glue to provide better mechanical strength, better cell distribution, and easier cell seeding, which would be expected to improve cartilage regeneration in vitro. Porcine cryo-precipitated fibrinogen and thrombin prepared from prothrombin activated by 10% CaCl2 solution were used in two groups. One is the fibrin glue group in which porcine chondrocytes were mixed with thrombin–fibrinogen solution, which was then converted into fibrin glue. The other is GHC6S-fibrin glue in which GHC6S particles were added into the thrombin-fibrinogen solution with porcine chondrocytes. After culturing for 1-2 weeks, the chondrocytes cultured in GHC6S-fibrin glue showed a round shape with distinct lacuna structure and showed positive in S-100 protein immunohistochemical stain. The related gene expressions of tissue inhibitor of metalloproteinases-1, matrix metalloproteinase- 2, MT1-MMP, aggrecan, decorin, type I, II, X collagen, interleukin-1 b, transforming growth factor-b 1 (TGF-b1), and Fas-associating death domain were checked by real-time PCR. Total GAGs and sulfated GAGs were also quantified by p-dimethylaminobenzaldehyde reaction and 1,9- dimethymethylene blue (DMMB) assay, respectively. The results indicated that the chondrocytes cultured in GHC6S-fibrin glue would effectively promote extracellular matrix (ECM) secretion and inhibit ECM degradation. The evidence could support that GHC6S-fibrin glue would be a promising scaffold for articular cartilage tissue engineering.
Subjects
fibrin
gelatin
hyaluronic acid
chondroitin-6-sulfate
articular cartilage
tissue engineering
Type
thesis
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