A bioluminescent method for measuring thymidylate kinase activity suitable for high-throughput screening of inhibitor
Journal
Analytical Biochemistry
Journal Volume
398
Journal Issue
2
Pages
269-271
Date Issued
2010
Author(s)
Hu C.-M.
Abstract
Blocking human thymidylate kinase (TMPK) function has a chemosensitization effect in anticancer treatment. However, a rapid and sensitive TMPK activity assay method suitable for inhibitor screening has been lacking. We have designed a luciferase-coupled TMPK assay in which luminescence emission is proportional to the magnitude of TMPK inhibition. The advantages of using this new method over the conventional nicotinamide adenine dinucleotide (reduced form, NADH)-coupling method in screening inhibitor include low cost, low limit in detecting inhibitory signal, more accurate, and devoid of interference due to compound absorbance at 340 nm.
SDGs
Publisher
Academic Press Inc.
Type
journal article