Wild bitter melon alleviates dextran sulphate sodium-induced murine colitis by suppressing inflammatory responses and enhancing intestinal regulatory T cells
Journal
Journal of Functional Foods
Journal Volume
23
Pages
590-600
Date Issued
2016
Author(s)
Abstract
The anti-inflammatory and immunomodulatory effects of wild bitter melon (WBM) on inflammatory bowel disease were investigated by using dextran sodium sulphate (DSS)-induced colitis murine model. WBM intake significantly improved weight loss, disease activity index and colon shortening. Colonic interleukin (IL)-6 and IL-1β were significantly decreased, and the anti-inflammatory cytokine IL-10 was increased in WBM-fed mice. The mRNA expression of transforming growth factor (TGF)-β and Foxp3 in colon, and Foxp3+ regulatory T (Treg) cell numbers in the mesenteric lymph nodes (MLN) were also significantly higher in the WBM group. Furthermore, ethyl acetate extract (EAE) and ethanol extract (EE) of WBM inhibited IL-6 secretion by lipopolysaccharide (LPS)-stimulated peritoneal macrophages. The n-butanol extract (BE) increased Treg cells differentiated from CD4+ T cells. This study suggested that the suppressive effect of WBM on colitis was contributed by the multiple bioactive components, at least, to inhibit inflammation and promote the development and activity of Treg cells. © 2016 Elsevier Ltd.
Subjects
Bitter melon; Colitis; Foxp3; Momordica charantia; Regulatory T cell
SDGs
Type
journal article