以高能輻射研製燒燙傷傷口暫時性敷料─燒燙傷傷口暫時性敷料之細胞毒性及生物商應性分析
Date Issued
1999-07-31
Date
1999-07-31
Author(s)
林輝
DOI
883011B002003NU
Abstract
A novel method of preparation of easily stripped off temporary wound dressing
material is disclosed. A tri-layer membrane system for artificial skin is prepared in
this study. In this process, the N-isopropyl acrylamide monomer is successfully
grafted on the non-woven fabric by copolymerization. It is initiated by plasma to
activate the surface of the non-woven cloth. N-isopropyl acrylamide is then grafted
onto the surface of the non-woven cloth by -ray irradiation. The last layer but the
most important of a bovine gelatin with glycosaminoglycans (chondroitin-6-sulfate) is
grafted by UV light, which serves as a matrix for the infiltration of fibroblasts,
macrophages, lymphocytes, and capillaries derived from the wound bed.
The goal in this work is to provide such a nonantigenic membrane closely
resembling dermis in its anatomic structure and chemical composition, which would
act as a scaffolding inducing the synthesis of a new dermis. The following describes
the construction and animal testing of this artificial skin in extensively damage. In the
experiment, the specimen are divided into 4 groups: (1) controlled group without
dressing material, (2) non-woven fabric, (3) non-woven fabric grafted with NIPAAm,
and (4) non-woven fabric grafted with NIPAAm, bovine gelatin, and
glycosaminoglycans from bottom to top in sequence.
After operated for 6 weeks, both controlled group and non-woven fabric group
stayed in the proliferative phase where no epidermis or dermis structure has been
traced in the section. 6 weeks of postoperation, the third group has been healed
completely in the maturation phase. The wound site has been totally recovery with
normal dermis and epidermis structure around but the dressing material still stayed on
the wound site. In the group of the non-woven fabric grafted with NIPAAm, geltain,
and glycosaminoglycans, it has been recovery to the final stage of maturation phase.
The wound site has been totally recovery at the 4th week of postoperation. The
dressing material of the group fall off automatically from the wound site without any
damage to the skin after recovery. We believe the dressing material will have a great
potential in medical application in the near future
Publisher
臺北市：國立臺灣大學醫學工程學研究所
Type
report
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