Influence of Electroosmotic Flow on the Ionic Current Rectification in a pH-Regulated, Conical Nanopore
Date Issued
2015
Date
2015
Author(s)
Lin, Dong-Huei
Abstract
Due to the rapid progress in biomedical materials in the past decades, the application of nano devices in DNA sequencing and membrane separation of proteins has drawen the attention of researches in various fields. Inspired by this, we analyze theoretically the influence of solution pH on the ion current rectification (ICR) behavior in a pH-regulated nanopore. Different from those in previous studies, where the effect of electroosmotic flow (EOF) is neglected, we solve simultaneously the Poisson, Nernst-Planck, and Navier-Stokes equations describing the behavior of a biomembrane, so the problem considered is closer to reality. Through adjusting the solution pH, ionic concentration, and the applied electric potential bias, we examine the ICR phenomenon under various conditions. The results of numerical simulation reveal that the influence of the EOF effect on ICR can be significant if the ionic concentration takes a medium high value, the solution pH is far from the isoelectric point, and the applied electric potential bias high. We show that the conventional result, where EOF is neglected, the percentage deviation in the rectification factor (RF) can be on the order of 100%. In addition, EOF is capable of influencing both qualitatively and quantitatively RF. Our analysis provides valuable information for designing experimental devices and selecting relevant parameters.
Subjects
pH-regulated
conical nanopore
electroosmotic flow
ionic current rectification
current rectification ratio
Type
thesis
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