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D型肝炎之病毒及免疫反應─利用噬菌體呈現法來尋找與D型肝炎病毒RNA交互作用的宿主因子(3/3)
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cellular factors interacting with hepatitis delta virus RNA
using phage-display cDNA library
cellular factors interacting with hepatitis delta virus RNA
using phage-display cDNA library
Date Issued
2000
Date
2000
Author(s)
李嘉哲
DOI
892315B002016MH
Abstract
Hepatitis delta virus (HDV)possesse
s several distinct biological activities such as autocatalytic cleavage and ligation activity,RNA-editing activity,hepatitis delta an
tigen
(HDAg)dependent replication transac
tivation and inhibition.
There should be some cellular factor
s interacting with HDV RNA
or HDAg to accomplish these activities.
The phage display technique utilizes
phages to express foreign proteins o
r peptides in fusion with one of their
coat protein.It is an
ideal tool to study protein-protein
interaction and protein-nucleic acid
interaction . We constructed
a T7 phage expression CDNA library f
rom a human liver and selected
with biotin-labeled genomic HDV
RNA probe.We obtained several
candidate genes including human
albumin,a leucine zipper protein,
apolipoprotein E,phosphodiesterase 3B
and some mitochondria-related proteins.
Only human albumin
stood the in vitro binding test
including northwestern and gel
mobility shift
assay.We are currently investigatin
g the biological meaning of this HDV RNA and human albumin interaction.
s several distinct biological activities such as autocatalytic cleavage and ligation activity,RNA-editing activity,hepatitis delta an
tigen
(HDAg)dependent replication transac
tivation and inhibition.
There should be some cellular factor
s interacting with HDV RNA
or HDAg to accomplish these activities.
The phage display technique utilizes
phages to express foreign proteins o
r peptides in fusion with one of their
coat protein.It is an
ideal tool to study protein-protein
interaction and protein-nucleic acid
interaction . We constructed
a T7 phage expression CDNA library f
rom a human liver and selected
with biotin-labeled genomic HDV
RNA probe.We obtained several
candidate genes including human
albumin,a leucine zipper protein,
apolipoprotein E,phosphodiesterase 3B
and some mitochondria-related proteins.
Only human albumin
stood the in vitro binding test
including northwestern and gel
mobility shift
assay.We are currently investigatin
g the biological meaning of this HDV RNA and human albumin interaction.
Subjects
Hepatitis delta virus
phage display
SDGs
Publisher
臺北市:國立臺灣大學醫學院內科
Coverage
計畫年度:89
第一期;起迄日期:1999-08-01/2000-07-31
第一期;起迄日期:1999-08-01/2000-07-31
Type
report
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Name
892315B002016MH.pdf
Size
24.22 KB
Format
Adobe PDF
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(MD5):8c1b7775b36a080e1fb35d6af0ce2045