Aptamer-conjugated polymeric nanoparticles for the detection of cancer cells through "turn-On" retro-self-quenched fluorescence
Journal
Analytical Chemistry
Journal Volume
87
Journal Issue
9
Pages
4925-4932
Date Issued
2015
Author(s)
Abstract
We have developed a simple, sensitive, and rapid fluorescence assay for the detection of cancer cells, based on "turn-on" retro-self-quenched fluorescence inside the cells. 1,3-Phenylenediamine resin (DAR) nanoparticles (NPs) containing rhodamine 6G (R6G) are conjugated with aptamer (apt) sgc8c to prepare sgc8c-R6GDAR NPs, while that containing rhodamine 101 (R101) are conjugated with TD05 for the preparation of TD05-R101DAR NPs. The sgc8c-R6GDAR and TD05-R101DAR NPs separately recognize CCRF-CEM and Ramos cells. The fluorescence intensities of the two apt-DAR NPs are both weak due to self-quenching, but they increase inside the cells as a result of release of the fluorophores from the apt-DAR NPs. The apt-DAR NPs' structure becomes less compact at low pH value, leading to the release of the fluorophores. The sgc8c-R6GDAR and TD05-R101DAR NPs allow detection of as low as 44 CCRF-CEM cells and 79 Ramos cells mL-1, respectively, using a commercial reader within 10 min. Practicality of the two probes have been validated by the quantitation and identification of CCRF-CEM and Ramos cells spiked in blood samples through conventional fluorescence and flow cytometry analysis, with advantages of sensitivity, selectivity, and rapidity. ? 2015 American Chemical Society.
SDGs
Other Subjects
Computational electromagnetics; Cytology; Diseases; Fluorescence; Fluorophores; Nanoparticles; Blood samples; Flow cytometry analysis; Fluorescence assay; Fluorescence intensities; Nanoparticle (NPs); Polymeric nanoparticles; Rhodamine 101; Self-quenching; Cells; aptamer; nanoparticle; polymer; 3T3 cell line; animal; cell separation; chemistry; fluorescence; human; mouse; Neoplasms; pathology; procedures; tumor cell culture; Animals; Aptamers, Nucleotide; Cell Separation; Fluorescence; Humans; Mice; Nanoparticles; Neoplasms; NIH 3T3 Cells; Polymers; Tumor Cells, Cultured
Type
journal article