Application of Mild Strain for Cross Protection and Culture Filtrate Derived from Soil Microorganisms on Management of Disease Caused by Cucumber mosaic virus

Cucumber mosaic virus (CMV) is an economically important plant viruses with a worldwide distribution and a very wide host range. We aim to use two strategies to manage disease caused by CMV on crops. One is to develop cross protection by utilization of mild strain virus and the other is to induce plant resistance in plant by treatment of culture filtrate derived from fungi (NTU1F8) isolated from soil. We first conducted our strategies on Nicotiana benthamiana. The result showed that 2b gene-partial deleted CMV induced only mild symptom on N. benthamiana and protect plant from infection by different severe strains of CMV. In addition, the treatment of culture filtrate of NTU1F8, can obviously decrease infection rate, delay symptom development and decrease virus accumulation. We also found pretreatment of N. benthamiana and Brassica juncea with culture filtrate of NTU1F8 following inoculation of Turnip mosaic virus could also decrease the infection rate and disease severity.