Using BLAST in molecular species identification of fungi 2: Gliomastix roseogrisea (Ascomycota, Hypocreales) as example for in-depth identification check
Journal
NOVA HEDWIGIA
Journal Volume
116
Journal Issue
1-2
Pages
137
Date Issued
2023
Author(s)
Abstract
BLAST (Basic Local Alignment Search Tool) is most commonly used for molecular species identification based on DNA sequences in fungi. Sequences labeled with wrong species names are multiplied in databases through a snowball effect, while the few correct information may eventually become hidden. By in-depth analyses such errors can be resolved, but the details of such analyses are not explained in the literature. Based on our own experience concerning the confusion of Gliomastix murorum and G. roseogrisea with BLAST searches, we suggest consulting taxonomic revisions for resolving problematic taxa. The confusion in Gliomastix was caused by usage of the nuclear internal transcribed spacer (ITS) rDNA barcode but lack of G. roseogrisea in one revision, and the presence of G. roseogrisea and use of large subunit ribosomal RNA gene sequences instead of ITS sequences in another parallel revision. Consulting phylogenetic revisions after BLAST searches is particularly recommended when DNA data of typified materials are in-complete or barcodes have been applied that preceded the present prevalent ITS rDNA. Although Gliomastix species are common and widespread, other than rDNA barcode sequences are extremely scarce. The distribution data of G. roseogrisea were clarified based on our revision.
Subjects
barcode gap; Bionectriaceae; data verification; RPB2; voucher deposit
Publisher
GEBRUDER BORNTRAEGER
Type
journal article