Staining of extracellular polymeric substances and cells in bioaggregates
Journal
Applied Microbiology and Biotechnology
Journal Volume
75
Journal Issue
2
Pages
467-474
Date Issued
2007
Author(s)
Abstract
Multiple fluorochrome experiments with as many fluorochromes as possible are desired for exploring the detailed structure of bioaggregates. Spectral peak interference and other practical limitations, however, restrict the maximum number of stains used simultaneously to three. This current study proposes a sixfold labelled scheme to stain the total cells, dead cells, proteins, lipids, and £\- and £]-polysaccharides in bioaggregates. Two aerobic granule systems, the phenol-fed and the acetate-fed granules, were utilized as the testing samples for demonstrating the use of the proposed scheme. ? 2007 Springer-Verlag.
Subjects
CLSM
Crosstalk
EPS
Staining
Type
journal article