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Initial artificial induction on the influence of ovary development of Japanese eel (Anguilla japonica)
Date Issued
2015
Date
2015
Author(s)
Huang, Yan-Rong
Abstract
The Japanese eel (Anguilla japonica), cannot complete sexual maturation spontaneously under condition of cultivation, and stay in previtellogenic stage, therefore the methods for induction by exogenous sex hormone for gonad development is required. This experiment using freshwater cultivated Japanese eel, 400~550 g in bodyweight, acclimated in seawater in one week, before artificial induction .The exogenous hormones are divided into salmon pituitary extracts (SPE) alone, SPE with MT and SPE with 11- KT , weekly artificially induced, divided into one-week and three-week treatment group. We use JB-4 resin section, and using oocyte stage classification, to observe (1) before and after artificial induction and (2) two androgens effects on ovary characteristics of Japanese eel. The results found SPE induced Japanese eel ovarian follicles, compared to without artificially induction, oil droplets and cortical alveoli significantly increased in ooplasm, and thickening of the outer layer of follicle cells, most advanced oocytes enter the oil droplet stage, showing artificial induction can promote Japanese eel oocytes development. SPE and two androgens effect, in SPE+11-KT treatment group cumulative amount of oil droplets in ooplasm are more than SPE and SPE+MT treatment groups in one-week treatment , but in three week treatment groups, no significant differences are observed between two androgen groups. From the above results, suggest artificial induction can allow ovarian follicles further develop, and two androgen effects show11-KT effects on ovarian follicles in the cumulative of oil droplets are faster than MT effects, shows that androgen are important for ovarian development of Japanese eel.
Subjects
artificial induction
androgen
Japanese eel
previtellogenic
JB-4 resin sections.
Type
thesis
File(s)
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Name
ntu-104-R01241213-1.pdf
Size
23.54 KB
Format
Adobe PDF
Checksum
(MD5):2fc4a9ce6cd631f764ad62726b2cee84