Antibody detection of SARS-CoV spike and nucleocapsid protein
Journal
Biochemical and Biophysical Research Communications
Journal Volume
314
Journal Issue
4
Pages
931 - 936
Date Issued
2004
Author(s)
Yen-Ta Lu
Shin-Tsung Ho
Chao-Chih Wu
Tsai-Yin Wei
Chia-Ju Chen
Yun-Ting Hsu
Po-Chen Chu
Ching-Hsin Chen
Jien-Ming Chu
Ya-Lin Jan
Chia-Chien Hung
Chi-Chen Fan
Yuh-Cheng Yang
Abstract
Early detection and identification of SARS-CoV-infected patients and actions to prevent transmission are absolutely critical to prevent another SARS outbreak. Antibodies that specifically recognize the SARS-CoV spike and nucleocapsid proteins may provide a rapid screening method to allow accurate identification and isolation of patients with the virus early in their infection. For this reason, we raised peptide-induced polyclonal antibodies against SARS-CoV spike protein and polyclonal antibodies against SARS-CoV nucleocapsid protein using 6x His nucleocapsid recombinant protein. Western blot analysis and immunofluorescent staining showed that these antibodies specifically recognized SARS-CoV. © 2004 Elsevier Inc. All rights reserved.
SDGs
Type
journal article