Solid-State NMR Determination of Protein Secondary Structure
Date Issued
2006
Date
2006
Author(s)
Mou, Yun
DOI
en-US
Abstract
This work focused on developing solid-state NMR pulse sequence to determine the protein secondary structure. Chapter 1 gives a basic view of using solid-state NMR to obtain the structure information. The main thesis was discussed in four parts: Ch.2: R-TOBSY: isotropic polarization transfer in biological solids. Ch.3: MCSP: Multiple Chemical Shift Precession for selective polarization transfer. Ch.4: psi angle determination: using correlation between C' CSA and Ca−Ha tensors.
Ch.5: CONGEE: Correlation Of Non-Gamma Encoded Experiment.
Each part is self-contained. The successful experiments of R-TOBSY (Chapter 2) and MCSP (Chapter3) in biological solids provide a crucial step for the psi angle determination in Chapter 4 and Chapter 5. Chapter 4~5 discussed the spin physics of the RACO experiment originally introduced by Ishii and co-workers in 1996. Through the analytical analysis, the optimal RACO experiment was designed and was employed for determination of the dihedral angles of L-alanine and DL-NAV. Brief conclusion and outlook were summarized in chapter 6.
Subjects
固態核磁共振
脈衝序列
蛋白質二級結構
扭轉角
solid-state NMR
pulse sequence
protein secondary structure
torsion angle
Type
thesis
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