Combination of multiphoton and reflective confocal imaging of cornea
Journal
Microscopy Research and Technique
Journal Volume
71
Journal Issue
2
Pages
83-85
Date Issued
2008
Author(s)
Abstract
We combine reflective confocal microscopy with multiphoton microscopy to form a minimally invasive technique to observe the cornea. The two imaging modalities allow detection of complementary information from the cornea. The autofluorescence signal shows the cytoplasm of epithelial cells, and the second harmonic generation signal is used to detect collagen, found mostly in the stroma of the cornea. The reflective confocal imaging allows detection of epithelial cells and keratocytes in the stroma. The system is first tested on bovine cornea. Assessment of the result on the bovine eye will be used to evaluate the potential of the system as a technique for in vivo clinical application. © 2007 Wiley-Liss, Inc.
Subjects
Confocal microscopy; Multiharmonic generation; Multiphoton processes; Ophthalmology; Three-dimensional microscopy
Other Subjects
collagen; animal tissue; article; autofluorescence; cell ultrastructure; confocal microscopy; cornea cell; cornea stroma; cow; epithelium cell; imaging system; minimally invasive surgery; multiphoton microscopy; nonhuman; priority journal; Animals; Cattle; Cornea; Corneal Stroma; Epithelial Cells; Image Processing, Computer-Assisted; Keratinocytes; Microscopy, Confocal; Bovinae
Type
journal article