Development and invagiation of a new injectable calcium phosphate cement for the treatment of peri-implant bony defects : Animal study
Date Issued
2014
Date
2014
Author(s)
Yang, Shu-Gen
Abstract
Particulated calcium phosphate has become more popular in clinical application in implant-related bone augmentation. The advantages of particulate form of bioceramics is owe to good ostoconductive efficiacy and easily gap filling, however, such particlulated bioceramics is lack of strength in space maintaineance which lead to unpredicated outcome in repair of intraoral large bony defect, such as peri-implant bone defect which heavy occlual loading may be encountered. For this reason, our team try to develop a new injectable bone cement based on α tricalcium phosphate with initial high flowability and subsequent hardness which can be applied intraorally. Furthermore, investigation of functional efficiacy based on canine animal model for such new cement in bone regeneration around large peri-implant defect have also been performed.
One Beagle and three Taiwanese hybrid dogs weighing between 7 kg to 10 kg were enrolled in this research protocol. According reseach protocol, the implant with defect can be attributed into four groups, the experimental group (n = 11), bone graft material used is a composite α tricalcium phosphate bone cement with monocalcium citrate; bone graft material in the control group (n = 6) used for the β tricalcium phosphate, hydroxyapatite and type I collagen; blank control group (n = 3) are blank group without graft insertion. The way is to experiment dogs around 4 premolar and the first molar of mandible, after six weeks of wound healing, the present study three groups of different materials and explants arbitrarily assigned according to different points in time, planting mandible in experimental dogs left and right sides, is the first major surgical approach in experimental animals toothless ridge good preparation certain size bone defects , then place the implant placement and bone graft material . There are four time points surgery, namely week 0, 7 weeks, 11 weeks and 15 weeks, the surgery will be performed while the implant resonant frequency and stability Periotest measurement and subcutaneous injected bone dye . Finally, at the sacrificed of the 19 weeks, followed by the use of the implant stability quotient (ISQ) analysis of the stability of the implant Periotest (PTV) analysis, radiographic analysis, tomography analysis, biopsy interpretation , fluorescence labeling was observed with bone analysis , etc. bone around the implant causing bone graft material level of life.
Results of the experimental group (compound α tricalcium phosphate with monocalcium citrate bone cement) are significantly different (P = 0.008) for the initial stability (ISQ value) of the material, but the material during wound healing compared with control group (hydroxyapatite complex, β tricalcium phosphate and collagen) easily exposed (group 4 weeks 25%, 100% of group 8 weeks, 12 weeks group 33.33% ) , 8 weeks of the experimental group compared to the stability of the implant ISQ values of the control group (P = 0.005) had a poor performance at other time points no significant difference PTV values (P = 0.043) . In 8 weeks group at different time points following the experimental group 12 weeks ISQ circumferential value (P = 0.012) compared to, PTV values (P = 0.044) had poorer performance values PTV group 8 weeks 4 weeks compared to group (P = 0.026) and bone mass tomography (Micro-CT bone volume) (P = 0.046) poor performance, there was no significant difference at other time points. Also in the fluorescein-labeled bone section shows the experimental group (compound α tricalcium phosphate with monocalcium citrate bone cement) with heavy lateral subperiosteal reaction and bone regeneration lead to widen of the ridge rather different from the control group and blank group.
Based on these findings of the results, we thus concluded that we have developed a new injectable α-tricalcium phosphate bone cement with initial high flowability and subsequent hardness which maybe a pontenial bone graft material intraorally. Furthermore, functional investigation on such new cement revealed prolonged resroption time and compromised results indicated composition medication may be done for the meet of needs in dental application in the future.
Subjects
α型磷酸三鈣
磷酸鈣骨水泥
可注射式
植體周圍骨缺損
動物實驗
Type
thesis
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