Statistical optimization of medium components for the production of Antrodia cinnamomea AC0623 in submerged cultures
Resource
Applied Microbiology and Biotechnology 72 (4): 654-661
Journal
Applied Microbiology and Biotechnology
Journal Volume
72
Journal Issue
4
Pages
654-661
Date Issued
2006
Date
2006
Author(s)
Abstract
The nutritional medium requirement for biomass and triterpenoid production by Antrodia cinnamomea AC0623 strain was optimized. Box-Behnken was applied to optimize biomass and triterpenoid production. According to response surface methodology (RSM), the optimum concentrations of N-source were determined. The results indicate that when a submerged culture in shake flasks was operated at 28°C, initial pH 5.5, and rotation speed 105 rpm, the biomass and triterpenoid content in dry basis could be increased to 3.20% (w/w) and 31.8 mg/g, respectively. The experiments were further scaled up to 100- and 700-l fermentors. Higher content of triterpenoids (63.0 mg/g) was obtained in 700-l fermentations by means of the control of cultural conditions and the modification of medium composition based on the RSM. © 2006 Springer-Verlag.
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Type
journal article
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