Antrodia cinnamomea fruiting bodies extract suppresses the invasive potential of human liver cancer cell line PLC/PRF/5 through inhibition of nuclear factor κB pathway
Journal
Food and Chemical Toxicology
Journal Volume
45
Journal Volume
45
Journal Issue
7
Journal Issue
7
Pages
1249-1257
Start Page
1249
End Page
1257
ISSN
02786915
Date Issued
2007-07
Author(s)
Hsu, Ya-Ling
Kuo, Po-Lin
Cho, Chien-Yu
Ni, Wen-Chiu
Tzeng, Tz-Fei
Kuo, Yueh-Hsiung
Lin, Chun-Ching
Abstract
In this study, we first report the anti-invasive effect of ethylacetate extract from Antrodia cinnamomea (EAC) fruiting bodies in the human liver cancer cell line PLC/PRF/5. Treatment with EAC decreased the cancer invasion of PLC/PRF/5 cells in a dose-dependent manner. This effect was strongly associated with a concomitant decrease in either the level or activity of VEGF, MMP-2, MMP-9 and MT1-MMP, and an increase in the expression of TIMP-1 and TIMP-2. EAC inhibited constitutively activated and inducible NF-κB in both its DNA-binding activity and transcriptional activity. Furthermore, EAC also inhibited the TNF-α-activated NF-κB-dependent reporter gene expression of MMP-9 and VEGF, and the invasion of cancer cells. EAC also exhibited an inhibitory effect on angiogenesis in a Matrigel Plug Angiogenesis Assay. Further investigation revealed that EAC's inhibition of cancer cell growth and invasion was also evident in a nude mice model. Our results indicate that EAC inhibits the activation of NF-κB, and may provide a molecular basis for drug development using EAC as an anti-invasive agent in the prevention and treatment of cancer.
Subjects
Antrodia cinnamomea
Invasion
Liver cancer
MMPs
NF-κB
SDGs
Other Subjects
acetic acid ethyl ester; Antrodia cinnamomea extract; gelatinase A; gelatinase B; immunoglobulin enhancer binding protein; matrigel; matrix metalloproteinase 14; natural product; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase 2; tumor necrosis factor alpha; unclassified drug; vasculotropin; angiogenesis; animal experiment; animal model; antineoplastic activity; Antrodia cinnamomea; article; cancer cell culture; cancer inhibition; cancer invasion; cancer model; controlled study; drug DNA binding; fruiting body; genetic transcription; human; human cell; male; mouse; nonhuman; nude mouse; Acetates; Antineoplastic Agents, Phytogenic; Carcinoma, Hepatocellular; Cell Line, Tumor; Dose-Response Relationship, Drug; Drug Screening Assays, Antitumor; Fruiting Bodies, Fungal; Humans; Liver Neoplasms; Matrix Metalloproteinase 14; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Neoplasm Invasiveness; NF-kappa B; Plant Extracts; Polyporales; Solvents; Tissue Inhibitor of Metalloproteinase-1; Tissue Inhibitor of Metalloproteinase-2; Tumor Markers, Biological; Vascular Endothelial Growth Factor A; Antrodia cinnamomea; Mus musculus
Type
journal article